You performed a Bradford assay with BSA (bovine serum albumin) as the standard to determine the concentration of your unknown protein. You obtained the graph below for your data. a. The absorbance of your protein sample was $0.643$ . What is the concentration of your sample? b. Assume the protein sample from part a) was prepared by taking $2 ? L$ of your unknown protein stock and diluting it with DI water to a total volume of $100 ? L$ . What is the actual concentration of your original unknown protein?

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You performed a Bradford assay with BSA (bovine serum albumin) as the standard to determine the concentration of your unknown

You performed a Bradford assay with BSA (bovine serum albumin) as the standard to determine the concentration of your unknown protein. You obtained the graph below for your data. a. The absorbance of your protein sample was

0.643

. What is the concentration of your sample? b. Assume the protein sample from part a) was prepared by taking

2 ? L

of your unknown protein stock and diluting it with DI water to a total volume of

100 ? L

. What is the actual concentration of your original unknown protein?

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