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(Solved): What is the major benefit of M13-based phage display with the scFv phagemid? The phage attaches to ...
What is the major benefit of M13-based phage display with the scFv phagemid? The phage attaches to the surface of the host strain and expresses the antibody fragment, essentially making a bacterium-phage-antibody. The phage lyses the infected bacteria releasing the antibody fragments. The phage promoter is very strong and causes the bacteria to expend \( 10 \% \) of their protein production on the antibody. The antibody fragment is expressed on the phage and the gene is in the packaged genome. The lysogenized bacteria will express the antibody molecule on their surface.