(Solved): These scientists showed the impact of a phosphorylated Serine 10 on the structure. Our research will ...

These scientists showed the impact of a phosphorylated Serine 10 on the structure. Our research will test how the phosphorylation of Serine 10 directly impacts CSP function and neuronal health. In the laboratory, we have developed a mutated CSP protein, that instead of a serine at position 10, it has ar alanine, referred to as CSPsion. 3. Will the mutant ${\mathrm{CSP}}^{510\mathrm{A}}$ still be phosphorylated by Protein Kinase A? (short answer, 4 points) 4. Do you predict a neuron expressing the mutant CSPS10A will be healthy or show signs of neurodegeneration? (short answer, 4 points) Neurodegeneration Cysteine string protein (CSP) is a molecular chaperone that is highly expressed in neurons. Impaired CSP function leads to neurodegeneration in humans and model organisms, but the mechanisms are currently unknown. Mammalian CSP must by phosphorylated on Ser 10 by Protein Kinase A to carry out the functions within the neurons. These scientists found Ser10 phosphorylation induces a conformational change in CSP. Figure 1. A model of the 3D structure of CSP conformational change following phosphorylation. CSP in the unphosphorylated state (figure $1\mathrm{A}$ and Figure $1\mathrm{C}$ ) and the phosphorylated state (figure $1\mathrm{B}$ and 1D). Blue represent areas of positive charge and red represents negative charge. Arrow points to exposed positively charged residues following phosphorylation. (Patel, 2016) Phosphnarylation of Serine 10