(Solved): The last reaction of glycolysis is catalyzed by the enzyme pyruvate kinase. The reaction is shown ...

The last reaction of glycolysis is catalyzed by the enzyme pyruvate kinase. The reaction is shown below Reaction 1 Pyruvate kinase H? Mg²+, K+ H?C OH ADP ATP OH OH Phosphoenolpyruvate Pyruvate i. The AG" of this reaction is -31.4 kJ/mole. Given this information, what is the AG" of hydrolysis of phosphoenolpyruvate ? Phosphoenolpyruvate + H?0 -> pyruvate + Pi AG'°=? ii. Mg²+ is a required cofactor for this reaction. What role does the Mg²+ play? iii. What is the K'eq of the reaction shown above? Does this seem readily reversible under biochemical standard conditions? Why or why not?