(Solved): The figure below shows a metabolic pathway that occurs in a microbe Z. sp with enzymes E1 - E8 and ...

The figure below shows a metabolic pathway that occurs in a microbe Z. sp with enzymes E1 - E8 and membrane transporter $\mathrm{T}$ that exchanges $\mathrm{X}$ and $\mathrm{Z}$. Membrane permeable substrate $A$ is added at high concentration for metabolic flux through the system. All metabolites are membrane permeable except $\mathrm{X}$ and $\mathrm{Z}$. Intermediates $\mathrm{D},\mathrm{J}$ and $\mathrm{H}$ are essential for microbe survival. Orange arrows indicate feedback allosteric inhibition of target enzymes. You wish to maximize production of end-product $\mathrm{X}$ with minimal $\mathrm{J}$ and $\mathrm{H}$. To achieve this goal you are able to make two enzyme modifications and two metabolite additions. Choose these options that together will most effectively enhance output of $X$ : 1. Select auxotrophic mutant of enzyme: 2. Create a mutant of enzyme 3. supplement with a metabolite: that is insensitive to allosteric inhibition. and a second metabolite: