(Solved): The figure above shows a restriction map of the A and S alleles, and the location of three poss ...

The figure above shows a restriction map of the ${?}^{\mathrm{A}}$ and ${?}^{\mathrm{S}}$ alleles, and the location of three possible DNA probes that could be used in Southern blot analyses (red shaded bars). The figure also shows a pedigree. I-1 and I-2 do not have sickle cell disease. They have four children who have not yet been diagnosed for sickle cell disease. DNA samples from the parents and the four children were digested with the enzyme EcoRI and analyzed by Southern blot analysis using only one probe. The results of the analysis are shown on the gel below the pedigree. Based on the Southern blot results, how many of the six children should be diagnosed with sickle cell disease?