(Solved): Task 3: Agarose Gel Electrophoresis Your amplicon from PCR was subjected to AGE for analysis. You ar ...

Task 3: Agarose Gel Electrophoresis

Your amplicon from PCR was subjected to AGE for analysis. You are shown the image of the gel loaded with the following lanes: (A) negative control, (B) size ladder (1, 2, 3, 4, 6, and 10 kb), (C) gDNA extract, (D) PCR amplicon. However, due to mishaps while loading the gel with the samples, you are not sure which lane is which. You are shown a diagram of the obtained gel below.

Label each lane of the gel. Write only the corresponding letters in the wells above.

Above each band in the size ladder, write its size (in kb).

Approximate the size (in kb) of the polystyrenase gene. Write your answer above the band corresponding to the gene.