(Solved): Question 1 and 2 , thank you. Pre-Lab Questions There are multiple SNPs in the TAS2R38 gene. For ...

Question 1 and 2 , thank you.

Pre-Lab Questions There are multiple SNPs in the TAS2R38 gene. For this experiment we are analyzing the SNP location at nucleotide position(np) 145 Sequence: Taster (wild-type): GGCGGCCACT........ Non-Taster (mutant): ..GGCGGGCACT ............... 1. The G-C polymorphism changes an mRNA codon from CCA in a Taster to GCA in a Non-Taster. What amino acid is present in a TASTER? In a NON-TASTER? What type of mutation is this? (silent/missense/nonsense) The primers used in this experiment will target the TAS2R38 gene, and the PCR product will include the SNP at np 145. The PCR product size is 221 base pairs long. Recognition sequence for the restriction enzyme HaeIII: GGCC Full fragment (uncut): 221 bp Cut: \( 44+177 \mathrm{bp} \) 2. Sketch the expected band pattern for the labeled genotypes on the gel below.