(Solved): Q3. The following data were obtained by HPLC chromatography on \( 15 \mathrm{~cm} \) _column. Consi ...

Q3. The following data were obtained by HPLC chromatography on \( 15 \mathrm{~cm} \) _column. Consider of the following equation of straight line: \[ Y=0.752 \mathrm{X}+0.075 \] a) Estimate the concentration of the unknown sample \( \mathrm{A} \) and \( \mathrm{B} \) by using internal standard method. A \( \mathrm{ppm} \) B \( \mathrm{ppm} \) b) Calculate the number of theoretical plates for the column; \( \mathrm{N} \) plate c) Calculate the average plate height for the column; \( \mathrm{H} \) \( \mathrm{cm} \) plate d) Calculate the resolution for \( \mathrm{B} \) and \( \mathrm{C}, \mathrm{R} \) :