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View the dilution in your lab manual for the turbidimetric assay to answer the following q ...
View the dilution in your lab manual for the turbidimetric assay to answer the following question In step \( 4,3 \mathrm{~mL} \) of \( E \). coli is transferred into \( 2 \mathrm{~mL} \) of sterile broth. What is the dilution? Select one: a. 1 b. \( 0.8 \) c. \( 0.2 \) d. \( 0.4 \) e. \( 0.6 \)
from bench 1 into the tube labeled "1" (this is the first dilution). Also transfer \( 1 \mathrm{ml} \) of \( E \). coli overnight culture into the cuvette labeled " 0. ." 4. Using a new pipette tip, transfer \( 1 \mathrm{ml} \) three times for a total of \( 3 \mathrm{ml} \) from tube "1" to the tube labeled "2," the second dilution. Mix well. 5. Using a new pipette tip, transfer \( 1 \mathrm{ml} \) twice for a total of 2 \( \mathrm{ml} \) from tube "2" to the tube labeled "3," the third dilution. Mix well. 6. Using a new pipette tip, transfer \( 1 \mathrm{ml} \) from tube "3" to the tube labeled " 4 ," the fourth dilution. Mix well. 7. Using a new pipette tip for each tube,