(Solved): Microbiology Lab Report: Acid Fast Staining & Endospore Staining 1. What is the objective of the Ac ...

Microbiology Lab Report: Acid Fast Staining & Endospore Staining 1. What is the objective of the Acid Fast lab? (1 point) To differentiate bactena into acid fast group non-acid fast groups. 2. Record your observations below for a mixed sample that was acid fast stained. (4 points) Sample Mixed sample Species A: coccus Species B: bacillus Other notes/observations: Objective Used BRS Total Magnification Color ASM Mic Library.org chenkel, Justice and Suchman Acid-Fast or Non-acid Fast? 3. Which dye is used for the Acid Fast stain? Why? (2 points) Carbol fushsin- helps the stain penetrate the celi wall. 4. Why do some procedures for the acid fast stain use heat? (1 point) Because the cell wall is so resistant to most Compounds. 5. If you were to isolate a random, unknown organism from the environment, which staining method would be better to start with: Gram staining or Acid fast staining? Why? (2 points) 6. What is the objective of the endospore lab? (1 point) differentiate bacterial spores from other vegetativ. cells to differentiate spore formers from non-spore formers. 7. Record your observations below for a sample that was endospore stained. (4 points) Clostridium species Other Sample notes/observations: Sample Bacillus subtilis Other notes/observations: Objective Used Objective Used ASM M Total Magnification ? Total Magnification Morphology Morphology Location of endospore Location of endospore