(Solved): - In performing a manual WBC count, you have filled the hemacytometer correctly but in removing the ...

- In performing a manual WBC count, you have filled the hemacytometer correctly but in removing the pipette from the groove, you jar the coverslip slightly The fluid in the hemacytometer does not appear to be disturbed. Should the counting procedure be performed on this charged hemacytometer? Why or why not? - Ms. Goodtech performs a manual WBC count. She counts 64 WBCs on side 1 and 56 WBCS on side 2 of the hemacytometer. Using a 1/100 dilution of blood, calculate the WBC count and identify as normal or abnormal. The usual WBC counting squares were used. - A technologist performs the weekly WBC count on John Jones who is receiving chemotherapy for cancer of the stomach. He has had leucopenia for the past three months. After diluting Mr. Jones' blood $1/20$, the number of cells counted on both sides of the hemacytometer is averaged; this is 32 cells. a) Calculate $\mathrm{Mr}$. Jones WBC count and b) define leukopenia. - A slightly turbid cerebrospinal fluid is diluted $1/10$ with saline, then the diluted CSF is loaded onto a hemacytometer and the entire ruled area is counted. An average of 81 WBCs are counted. Calculate the number of WBCs/ $?\mathrm{L}$ in the spinal fluid. Show calculations. - Ms. X performed the standard diluting process $(1/20)$ properly for a WBC manual count but in the counting process she counted 5 large squares instead of 4 squares. Side 1 had 160 cells and side 2 had 180 WBCs. Change your formula to compensate for her error and calculate the WBC count. Should this result be reported? Why or why not? - A manual RBC count is performed using a $1/200$ dilution. 410 cells are counted on side 1 of the hemacytometer. 430 RBCs are counted on side 2. Calculate the RBC count. - An MLT student performed a manual RBC count but when counting, only 4 small squares are used. The dilution was $1/400$. Revise the formula and calculate the correct RBC count. The average number of RBCs counted was 180 .