(Solved): (II) Applied problem using a standard curve Use the information given to draw a standard curve ...

(II) Applied problem using a standard curve Use the information given to draw a standard curve \& calculate the phosphate $(P)$ concentration in a $24h$ urine sample. (On the table below fill in the P concentration in the P standards) A patient excreted $1430\mathrm{ml}$ of urine in 24 hours. A $2\mathrm{ml}$ aliquot/sample of the urine was placed in a $100\mathrm{ml}$ volumetric flask, treated with $10\mathrm{ml}$ of molybdate reagent and $4\mathrm{ml}$ of aminonaphtholsulphonic acid reagent and diluted to the mark. A series of five colour standards was similarly prepared in which $0,1,2$, 3 and $4\mathrm{ml}$ of phosphate standard (containing $0.4\mathrm{mg}$ of $\mathrm{P}$ in $5\mathrm{ml}$ ) were used respectively. These were also diluted to $100\mathrm{ml}$ after addition of the molybdate and aminoaphtholsulphonic acid reagents. The absorption was measured in a spectrophotometer at $660\mathrm{nm}$. Calculation of GRAMS of phosphate excreted per 24 hours.