(Solved): Hypothesis Elimination You have a new line of mice that die very ently after birth showing signs o ...

Hypothesis Elimination You have a new line of mice that die very ently after birth showing signs of severe dehydration. You supplement their water intoke by injection large amounts of normal saline (osmotically balanced water) into the mice. Despite this large fluidi intake, the mice still die, but prior to dying, they release large amounts of dillute urine. Your job is to determine the mechanism for increased urine output. A. The mutant does not incorporate aquaporin 2 protelns on the apical membrane of collecting duct epithelial cells. 8. The sodium channel function is reduced in the collecting duct of mutant mice. C. The \( \mathrm{Na}^{+} / \mathrm{K}+ \) pump function is reduced in the coliecting duct of mutant mice D. Mutant mice fall to secrete vasopressin. Hypothesis testing time except in this version there can be more than one possiblity per experiment. Experiment one: Youf prepare memb cellular membranes from celis of the collecting duct from the following groups of mice: Wild Type Wild Type with Normal Saline supplement Mutant Mice- Mutant Mice with Normal Saline supplement Aralysis of a membrane preparation shows no difference in membrane proteins between mutant and wid type of between mutant and wild type that Were supplemented with Normat Saline. However, both groups the membrane proteins are differemt between supplemented and non-supplemented groups The mutant does not incorporate aquaporin 2 proteins on the apical membrane of collecting duct epithelial cells. The sodium channel function is reduced in the collecting duct of mutant mice. The \( \mathrm{Na}+/ \mathrm{K}+ \) pump function is reduced in the collecting duct of mutant mice. Mutant mice fall to secrete vasopressin. None can be eliminated. All can be eliminated. Hypothesis Elimination You have a new line of mice that die very early after birth showing signs of severe dehydration. You supplement their water intake by injection large amounts of normal saline (osmotically balanced water) into the mice. Despite this iarge fluid intake, the mice still die, but prior to dying, they release large. amounts of dilute urine. Your job is to determine the mechanism for increased urine output: A. The mutant does not incorporate aquaporin 2 proteins on the opical membrane of collecting duct epithellal cells, B. The sodlum channel function is reduced in the collecting duct of mutant mice. C. The \( \mathrm{Na}+\mathrm{K}+ \) pump function is reduced in the collecting duct of mutant mice. D. Mutant mice fall to secrete vasopressin. Hypothesis testing time except in this version there can be more than one possibility per experiment Experiment two: Using cultured cels from wild type and mutant mice. you measure the osmolanty of the extrocellular fluid, which would be equivalent to the interstitiol fluid, in these ideal conditions, there is no difference in osmolarity. You have a new line of mice that die very early affer birth showing signs of severe dehydration. You supplement their water intake by injection large amounts of normal saline (osmotically balanced water) into the mice. Despite this large fuld intake, the mice still die, but prior to dying, they release large amounts of dilute urine. Your job is to determine the mechanism for increased urine output. A. The mutant does not incorporate aquaporin 2 proteins on the apical membrane of collecting duct epithellal cells. 8. The soclum channel function is reduced in the collecting duct of mutant mice. C. The Nat/K+ pump function is reduced in the collecting duct of mutant mice. D. Mutant mice fall to secrete vasopressin. Hypothesis testing time except in this version there can be more than one possibuty per experiment. Experiment three: Next you cione and sequence the vasopressin receptor from mutant mice. The sequence is identical between wild type and mutant receptors. Hypothesis Elimination You have a new line of mice that die very earty affer bith showing signs of severe dehydration. You supplement their water intake by injection large amounts of normbl saline (osmotically balanced water) into the mice. Despite this large fluld intake, the mice still die, but prior to dying, they release iarge amounts of dillute urine. Your job is to deterimine the mechanism for increased urine output. A. The mutant does not incorporate aquaporin 2 proteins on the apical membrane of collecting duct epithellal cells. B. The sodium channel function is reduced in the collecting duct of mutant mice. C. The \( \mathrm{Na}+\mathrm{K}+ \) pump function is reduced in the collecting duct of mutant mice. D. Mutant mice fall to secrete vasopressin. Hypothesis testing time except in this version there can be more than one possiblity per experiment: Experiment one: Your prepare memb cellular membrenes from cells of the colfucting duct from the following groups of mice: Wild Type Wild Type with Normal Soline supplement Mutant Mice Mutant Mice with Normal Saline supplement Analysis of a membrane preparation shows no difference in membrane proteins between mutant and wild type or between mutant and wild type that were supplemented with Normal Saline. However, both groups the membrane proteins are different between supplemented and non-supplemented groups. The muthot does not incorporate aquoporin 2proteins on the apical membrane of collectirig duct epithelial cells: The sodium channel function is reduced in the collecting duct of mutant mice. The \( N a+i K+ \) pump function is reduced in the collecting duct of mutant mice. Mutant mice fall to secrete vasopressint. None can be eliminated. All can be eliminated