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(Solved): HITi-t15 cells (a hamster epithelial cell line) were permeabilised and incubated with propidium io ...
HITi-t15 cells (a hamster epithelial cell line) were permeabilised and incubated with propidium iodide. The cells were then analysed by flow cytometry (data below). 256 cell number A || fluorescence 1023 cell number 256 B III. fluorescen a. Which phase(s) of the cell cycle are represented by the peaks I, II and III. (3 marks) b. Which histogram (a or b) is more likely be from confluent cells? Explain your answer. (2 marks) 1023
Expert Answer
Concepts involved in solving the question: Cell culture can be of two types: synchronous when all the cells are in the same stage of the cell cycle and asynchronous when cells are in different stages of the cell cycle and the proportion of cells in d