(Solved): help please To determine these values, a well-controlled experiment must be conducted. A series of t ...

To determine these values, a well-controlled experiment must be conducted. A series of test tubes containing a set amount off buffer are shown below. The indicated concentration of substrate (specific to the enzyme being tested) is added to each tube as labeled. Equal amounts of enzyme are then added to each tube, and the amount of product is determined by spectrophotometry at a designated time-point (same for all). The initial velocity $\left({\mathrm{v}}_1\right)$ is determined for each sample and is shown in the chart below. Ne can plot these values and fit a zurve. This type of graph is called a Michaelis-Menten plot. From this graph, we can estimate $V_{\max }$ and $K_M$ for this enzyme-substrate reaction. ${\mathrm{V}}_{\max }$ : ${\mathrm{K}}_{\mathrm{M}}:$ Be sure to include the correct units for each and indicate on the graph how you estimated these values.