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(Solved): Experiment 1: The Effects of Enzyme ConcentrationAmylase is an important metabolic enzyme. Its funct ...



Experiment 1: The Effects of Enzyme Concentration

Amylase is an important metabolic enzyme. Its function is to catalyze the hydrolysis of starch into glucose. Based on our lab ppt and material: 

Methods: 

Tube 1: 6 mL starch + 6 mL amylase (full strength)

Tube 2: 6 mL starch + 4 mL amylase (2/3 strength) + 2 mL water

Tube 3: 6 mL starch + 2 mL amylase (1/3 strength) +4 mL water

Tube 4: 6 mL water + 6 mL amylase 

Tube 5: 6 mL starch + 6 mL water

The total volume for all reactions is 12 mL.

Take the sample from each tube and perform an iodine test every
minute.
8. Write the following content for experiment 1:
1) H
Take the sample from each tube and perform an iodine test every minute. 8. Write the following content for experiment 1: 1) Hypothesis: 2) Independent Variable: 3) Dependent Variable: 4) Positive control: 5) Negative control: Results Tube Material 2 3 5 6 mL starch +6 mL amylase 6 mL starch +4 mL amylase 6 mL starch +2mL amylase Color at 1 min Dark blue Dark blue Dark blue Color at 2 min 6 mL starch Dark +6 ml water blue Yellow Dark blue Dark blue Color at 3 min Dark blue Yellow Dark blue Dark blue Colorat 4 min Dark blue Yellow Dark blue Color at 5 min Yellow Yellow 6mL water+ Yellow Yellow Yellow Yellow Yellow 6ml amylase Yellow Dark blue Yellow Dark blue 9. How did amylase concentration affect the reaction rate? 10. Does the lowest enzyme concentration produce a reaction? 11. Interpret your results tube by tube, reach a conclusion and determine whether your results support or reject your hypothesis. O


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8. 1) Hypothesis - The presence of the amylase in the solution causes the hydrolysis of starch into glucose. 2) Independent variable - Amylase concentration 3. Dependent varia
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