(Solved): DNA methylation is involved in tomato ripening. Pyrosequencing is a method that allows for accurate ...

DNA methylation is involved in tomato ripening. Pyrosequencing is a method that allows for accurate quantitation of DNA methylation at cytosines within a given DNA fragment. Below is
pyrosequencing data that shows DNA methylation at multiple cytosine sites within the promoter region of Cnr, a gene involved in tomato ripening.

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a) Define and describe the term epigenetics. To help explain your answer provide a brief description or contextual example. (4 marks) DNA methylation is involved in tomato ripening. Pyrosequencing is a method that allows for accurate quantitation of DNA methylation at cytosines within a given DNA fragment. Below is pyrosequencing data that shows DNA methylation at multiple cytosine sites within the promoter region of \( \mathrm{Cnr} \), a gene involved in tomato ripening. b) Using the graph below, explain what happens to DNA methylation of the Cnr promoter region across tomato ripening. What will happen to \( C n r \) gene expression across the 4 stages of tomato ripening defined below? (8 marks) A B Figure 2: A) A photograph showing four (4) stages of tomato ripening (labelled 1-4). B) Graph showing the corresponding percentage DNA methylation at six (6) cytosine sites across the \( \mathrm{Cnr} \) promoter for each of the four (4) stages of tomato ripening.