(Solved): Diffraction of light observed during microscopy results in objects that are \( 200 \mathrm{~nm} \) ...

Diffraction of light observed during microscopy results in objects that are \( 200 \mathrm{~nm} \) or larger in reality all appearing to be a lot smaller, at \( 10.5 \mathrm{~nm} \) diameter when taking an image. is a term used to describe out-of-focus light, such that confocal microscopy that eliminates out of focus light allows nearly unlimited resolution. occurs due to constructive and destructive interference of light due to its properties as a wave. makes it so that use of red (wavelength \( \sim 650 \mathrm{~nm} \) ) light will allow better resolution than use of blue (wavelength \( \sim 470 \mathrm{~nm} \) ) light.