Consider the DNA sequence provided below. The transcription start site
(+1)
is indicated in bold.
5^(')dots
..TTA ATCTGAAGCA TGGAGCTATG TTCAGGTAAG TGCT
q,
A.
q,
TCAGGGCAA...
3^(')
a) Identify and underline the
5^(')
and
3^(')
splice sites. (1) b) Provide the mature mRNA sequence. (2) c) If the 5' splice site was mutated in the gene, what would the consequence be to the protein that is produced? (2) d) Where would the promoter sequence be found? (1) e) What would be the effect of a TBP mutation on the expression of this gene? (2)