(Solved): Biochemistry. please help!!! Describe the enzyme-catalyzed mechanism for a serine protease as depict ...
Biochemistry. please help!!!
Describe the enzyme-catalyzed mechanism for a serine protease as depicted in the schematic In STEP 1, a diffuses into the active site. The specificity for binding is found at deep pocket (not shown) that, for chymotrypsin, recognizes a and for trypsin recognizes a This places the peptide bond to be cleaved in the correct orientation and proximity for the catalytic triad
The oxyanionhole stabilizes the two tetrahedral intermediates that form at steps 2 and 6 during the hydrolysis of a peptide bond. Product 1 leaves at step 4 Substrate 2 diffuses in at step 5 Product 2 leaves after step 7 This chart summarizes the role of the catalytic triad residues at each of the steps 2-3 and 6-7: Amino acid Catalytic role in Step 2 Catalytic role in Step 3 Catalytic ro residue acts as a SER HIS