Based on the generalizable principles that you’ve learned from studying the lac operon, it’s time to design your own operon. In this scenario, you need an operon that can be used to regulate the transcription of genes encoding proteins that function in the metabolic pathway used to synthesize uracil from precursors present in the cell.
To help you get started, think about which of the operons we've examined is more similar to the operon you need to make.
when should this operon be turned on?
when should this operon be turned off? ?
should the default be on or off?
how many cis elements (binding sites in the DNA that can interact with positive or negative regulators) are needed to regulate your operon? ?