(Solved): As a fish geneticist, you have isolated a recessive mutation that alters the formation of colored ...

As a fish geneticist, you have isolated a recessive mutation that alters the formation of colored stripes along the body plan at the homozygous state. You name this mutation pitiful stripeless 1 (ptl1'). You'd like to determine if the PTL1 gene functions cell-autonomously to regulate stripe formation and opt for a genetic mosaic approach to answer this question. Your collaborator previously created a heterozygous $${PTL1}^{+}$$ptl1- fish carrying FRT sites on both the PTL1 - and ptl1- - carrying chromosome homologs, between the PTL1 locus and the telomere (drawing shown below; FRT sites represented by red triangles). This fish also carries a FLPase gene under the control of a heat-shock-inducible promoter, elsewhere in the genome. During fish development, you hope to create genetic mosaics with homozygous ptl1- pt/1- sectors surrounded by PTL1 $${}^{+}$$PTL1 $${}^{+}$$(twin sector) and $${\mathrm{PTL}}^{+}$$ptl1 $${}^{?}$$cells (initial genotype of the zygote) by providing a short pulse of heat shock, thereby triggering FLP-dependent recombination between FRT sites on homologous chromatids (see below). A. Is the fish line your received from your collaborator appropriate to generate homozygous pt/1- pt/1 sectors upon heat shock? Please explain your answer in 2 sentences or less. (6 points) B. If it is not appropriate, please suggest how you would modify it to allow this experiment (where would you place the FRT site relative to the PTL1 locus and the centromere?). Please explain your answer in 3 short sentences or less. (6 points)