Above is a kinetic scheme for an enzyme reaction.
Annotate the above schematic with rate constants (k₊₁, k_-1, k₊₂, k_-2).
Which rate constant is typically neglected in this schematic?
What assumption is required in this situation to obtain the expression k_obs from experiments measuring product formation vs time?
In the schematic drawn above the expression for k_obs can be simplified. Write out the simplified expression corresponding to the situation drawn above and explain why it is applicable?

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Above is a kinetic scheme for an enzyme reaction.

Annotate the above schematic with rate constants (k₊₁, k_-1, k₊₂, k_-2).

Which rate constant is typically neglected in this schematic?

What assumption is required in this situation to obtain the expression k_obs from experiments measuring product formation vs time?

In the schematic drawn above the expression for k_obs can be simplified. Write out the simplified expression corresponding to the situation drawn above and explain why it is applicable?

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