(Solved): Above are shown six nucleotides, labeled A-F. (B) 1a) List all nucleotides (A-F) that apply to each ...

Above are shown six nucleotides, labeled A-F. (B) 1a) List all nucleotides (A-F) that apply to each description below. (10) The nucleotide(s) above that is/are dCMP (A, B, C, D, E and/or F). The nucleotide(s) above that is/are used by DNA helicase. The nucleotide(s) above that is/are used by (bacterial) Primase. The nucleotide(s) above that is/are released by DNA polymerase proofreading activity when DNA polymerase erroneously inserts an A across from a C during replication. The nucleotides(s) above that is/are used in a Sanger sequencing reaction. 1b) You wish to perform an in vitro transcription reaction to generate an RNA with the sequence 5'-AACCA. i) List the nucleotide(s) above (A-F) that would be used in this transcription reaction. (2) ii) You wish to carry out the transcription reaction in a manner where the 5'-AACCA RNA is radioactively labeled with ${}_{32}\mathrm{P}$ at only one position. On the nucleotide(s) indicated in $1\mathrm{b}?\mathrm{i}$, circle one phosphorous atom (only one in total please) that could be ${}_3{}_{32}\mathrm{P}$ to ensure ${}_{32}\mathrm{P}$-labeling of the RNA at only one position. (2) Question 2. Below is shown a replication fork (DNA in thin grey, RNA in thick black). 2a) In the circle on the rioht indicate whether the arrow noints to a 5 ' or a 3 ' end? (2) 2b) Arrows a-i point to positions of nucleotides located at the end of individual molecules. For each of the nucleotide triphosphates below, list which position(s) (a-i) the nucleotide triphosphate is/are about to be added to by a polymerase: (4) A dNTP: About to be added to position(s) (a-i): An NTP: 2c) For each subset of nucleotides a-i listed below, list which of the subset was added most recently in the shown replication fork. (6) a, b: e, f, g: e, f, h, i: 2d) List the next nucleotide (a-i) that will be removed by a nuclease acting during replication assuming that the nuclease acting during normal replication is a 5'-to-3' exonuclease. (2) Question 3 On the right is shown a telomere being extended by telomerase in a new organism. 3a) In the indicated circle, list whether the arrow points to a 5' or a 3' end? (2) 3b) List the acronyms for all nucleotides you would need to add to a test tube with the shown Telomerase and DNA to achieve extension of the telomere (for example, ATP is an acronym for a nucleotide; do not list nucleotides not needed). (4) 3c) List the sequence of next 8 nucleotides added to the shown telomere. (4) 5' 3d) How long (in nucleotides) is the telomeric repeat of the shown telomere? (2)