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(Solved): A sample of an unknown peptide was divided into two aliquots. One aliquot was treated with trypsin ...
A sample of an unknown peptide was divided into two aliquots. One aliquot was treated with trypsin and the other was treated with cyanogen bromide. Given the following sequences ( N-terminal to C-terminal) of the resulting fragments, deduce the sequence of the original peptide. Trypsin treatment Glu-Met-Tyr-Asp-Thr-Pro-Arg Ala-Gin His-Glu-Asp-Met-Phe-Ale-Lys Ala-His-Ala-Arg Cyanogen bromide treatment Ala-His-Ala-Arg-Glu-Met Phe-He-Lys-Ala-Gin Tyr-Asp-Thr-Pro-Arg-His-Glu-Asp-Met Glu-Met-Tyr-Asp-Thr-Pro-Arg-His-Glu-Asp-Met-Phe-Ile-Lys-Ala-His-Ala-Arg-Ala-Gln Tyr-Asp-Thr-Pro-Arg-His-Glu-Asp-Met-Ala-His-Ala-Arg-Glu-Met-Phe-Ile-Lys-Ala-Gin Ala-Mis-Ala-Arg-Glu-Met-Tyr-Asp-Thr-Pro-Arg-Mis-Glu-Asp-Met-Phe-Ile-Lys-Ala-Gin Phe-lle-Lys-Ala-Gin-Ala-His-Ala-Arg-Glu-Met-Tyr-Asp-Thr-Pro-Arg-His-Glu-Asp-Met Ala-Gin-His-Glu-Asp-Met-Phe-lle-Lys-Glu-Met-Tyr-Asp-Thr-Pro-Arg-Ala-His-Ala-Arg His-Glu-Asp-Met-Phe-Hle-tys-Ala-His-Ala-Arg-Ala-Gin-Glu-Met-Tyr-Asp-Thr-Pro-Arg None of the Above