(Solved): 6. Draw a schematic diagram of an optical system design for flow cytometry measurements of cells st ...

6. Draw a schematic diagram of an optical system design for flow cytometry measurements of cells stained with antibodies conjugated with fluorescent dyes FITC and Texas Red: a. Show how lasers can be spatially arranged for simultaneous excitation of 2 dyes b. Incorporate FSC and SSC channels in the optical design c. Indicate transmission characteristics of all optical filters and dichroic mirrors allowing simultaneous excitation of 2 dyes, separation of the scattered light and the fluorescent light emitted by 2 dyes