(Solved): 2. You have a protein X and you want to know what its isoelectric point is. You run Protein X on a ...

2. You have a protein $$\mathrm{X}$$ and you want to know what its isoelectric point is. You run Protein $$\mathrm{X}$$ on a gel together with 5 other proteins whose isoelectric points (IEP) are known. You establish a standard curve for isoelectric points by measuring how far each protein has traveled on the gel and plotting this against their isoelectric point. Your standard curve is shown on the right. You then measured how far Protein $$\mathrm{X}$$ has traveled on the gel and determine it to be $$30\mathrm{mm}$$. What is the isoelectric point of Protein $$X$$ ? and phenol red) were plotted against their known isoelectric point (IEP). 3. Indicate all that apply. You have made an aqueous extract of photopigments and loaded it into a well in your gel, and you loaded Dye 1 and Dye 2 into the two adjacent wells. You see all samples at the bottom of the wells. You connected the leads, turned on the power unit and made sure it was set to $$120\mathrm{V}$$. You check after 1 hour but there are no bands on the gel or in the wells. Which of the following statements could explain what happened? a. You connected the leads wrong, red to black b. You did not cover the gel sufficiently with electrophoresis buffer c. You put in the gel with the well side towards the positive pole instead of the negative pole d. You did not push the leads in all the way, so no electricity flowed in the chamber although the power unit was on e. You forgot to hit the run button f. You loaded too much of your samples and dyes g. You forgot to add glycerol to the samples h. You thought you set the power unit to $$120\mathrm{V}$$, but it was $$120\mathrm{mV}$$ instead 4. Short answer. Explain your answers from above. What exactly would happen for each of these mistakes? What should you see on the gel?