(Solved): 2. Now you need to prepare a different buffer: 2.000L of 2.00M phosphate buffer, pH 7.40. You ...

2. Now you need to prepare a different buffer: $$2.000\mathrm{L}$$ of $$2.00\mathrm{M}$$ phosphate buffer, $$\mathrm{pH}$$ 7.40. You have the following reagents available to you. ( $$\mathrm{pKa}$$ 's for phosphoric acid are: $$2.14,7.20$$, and $$12.40$$ ) a. From the table above, select the combination of reagents that you will use for your buffer. (Note: there are several possibilities - you just need to choose one.) (1) b. Calculate how much of each reagent (and water) you will use to make this buffer. (5)