(Solved): 2. Draw the approximate elution profile (i.e. A280 vs. vol) obtained by passing a mixture of the f ...

2. Draw the approximate elution profile (i.e. A280 vs. vol) obtained by passing a mixture of the following proteins through a column of Sephadex G-100. The molecular weight is shown for each protein. Assume there are equal amounts of each protein in the mixture and that each protein has a similar extinction coefficient at 280 nm. (2 points) Molecular weight (Da) Protein Myoglobin Catalase 16900 222000 Cytochrome c 13370 Myosin 524000 Serum albumin 68500 Chymotrypsin 23240