(Solved): 1. The TLC plate developed by a student in ethyl acetate for a mixture of the following compounds ...

1. The TLC plate developed by a student in ethyl acetate for a mixture of the following compounds is given below. Answer the following questions. $$\mathbf{A}$$ B C $$40\%$$ Acetone in hexane A) Assign the identity of each spot on TLC. (2 pts) B) If you use $$80\%$$ acetone in hexane in place of $$40\%$$ acetone in hexane to develop the above mixture of compounds, which compound do you see eluting as the topmost spot on the TLC? (1 $$\mathbf{pt})$$ 2. Why do we need to filter the hexane solution of spinach extract through a Pasteur pipet packed with anhydrous sodium sulfate? (1 pt) 3. Indicate whether each one of the following statements regarding chromatography is either "True" or "False".(6 pts) A) A polar solvent will decrease the Rf value of all compounds. B) In TLC only compounds which are solid in nature can be analyzed. C) Thin layer chromatography is based on electrical mobility of ionic species. D) The component which is strongly adsorbed by the stationary phase will tend to move slowly in the mobile phase E) The minimum and maximum values for $$R_f$$ (retention factor) is 0 and 100 . F) TLC can be used to determine the purity of a compound.