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(Solved): 1- DNA profiles in this experiment require first PCR and then gel electrophoresis. What is the func ...




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1- DNA profiles in this experiment require first PCR and then gel electrophoresis. What is the function of each? 10 pts 2- What are the necessary reagents to perform PCR? Explain the reason why each of these are needed. 15 pts 3. What are STRs? Why are these sequences useful for this type of analysis? 5 pts 4- Explain what the temperature settings in the thermal cycler are not constant but go from 95 to 52 and then to 72 . Indicate what is achieved at each of these temperatures using the correct terminology. 20 pts 5- What explains the movement of DNA through an agarose gel? 5 pts 7- How is an allele ladder used in this experiment? 5 pts 8- How was a DNA profile achieved in this lab? Insert a labeled photograph of your group's gel. Insert a table that summarizes and explains the results (Allele ladder, CS genotype, Suspect 1-4 genotypes) 30pts 9- Does the Crime Scene DNA Sample have a genotype that matches any of the suspects? If so, which one matches? Which suspects were excluded? 5 pts 10- Would the DNA evidence as obtained in this lab be enough proof to incriminate a suspect? Why or why not? 5 pts


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